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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (N-Term) antibody

Details for Product No. ABIN372605, Supplier: Log in to see
Antigen
  • darpp32
  • darpp-32
  • Darpp-32
  • Darpp32
  • DARPP-32
  • DARPP32
  • AU040756
Epitope
N-Term
40
38
16
13
6
6
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
151
113
103
20
18
17
7
6
5
4
2
2
Host
Rabbit
155
13
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
131
56
47
28
24
17
5
4
3
1
1
Supplier
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Immunogen Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
Isotype IgG
Specificity Specific for the ~32k DARPP-32 protein.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.
Purification Affinity Chromatography.
Alternative Name DARPP32 (PPP1R1B Antibody Abstract)
Background DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
Gene ID 84152
NCBI Accession NP_001229393
UniProt Q9UD71
Research Area Signaling
Application Notes Western Blot: 1/1000. Immunohistochemistry: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (N-Term) antibody (ABIN372605) anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (N-Term) antibody
Background publications Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).