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MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (pThr202)

Details for Product anti-MAPK3 Antibody No. ABIN372623, Supplier: Log in to see
Antigen
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Alternatives
anti-Human MAPK3 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
pThr202, Tyr204
56
44
42
38
27
20
20
15
14
13
9
8
8
5
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
454
217
160
26
22
21
19
19
18
12
7
4
4
4
4
3
3
3
2
2
2
1
Host
Rabbit
314
152
21
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
23
23
21
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
379
187
156
96
67
58
47
33
32
18
10
4
3
2
2
2
1
1
Supplier
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.
Isotype IgG
Specificity Recognizes the ~42k-44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and ZebrafishSpecies reactivity (tested):Human and Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002, Ahn, 1993, Tanoue and Nishida, 2003, Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595
NCBI Accession NP_002737
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (pThr202) (ABIN372623) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (Tyr204), (pThr202) antibody
Background publications Tanoue, Nishida: "Molecular recognitions in the MAP kinase cascades." in: Cellular signalling, Vol. 15, Issue 5, pp. 455-62, 2003 (PubMed).

Johnson, Lapadat: "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases." in: Science (New York, N.Y.), Vol. 298, Issue 5600, pp. 1911-2, 2002 (PubMed).

Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002 (PubMed).

Ahn: "The MAP kinase cascade. Discovery of a new signal transduction pathway." in: Molecular and cellular biochemistry, Vol. 127-128, pp. 201-9, 1994 (PubMed).