GABRA1 antibody (Cytoplasmic Loop)

Catalog No. ABIN372626

Product Details anti-GABRA1 Antibody

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Binding Specificity: Cytoplasmic Loop

Reactivity: Rat, Human, Mouse, Cow, Dog, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~51k Alpha-1 subunit of the GABAA receptor in Western blots. Labeling is absent in Alpha-1 subunit knockout animals.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Canine and non-Human Primates.
Species reactivity (tested):Rat and Mouse.

Purification: Affinity Chromatography.

Immunogen: Fusion protein from the cytoplasmic loop of the Alpha-1 subunit of Rat GABAA receptor.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-GABRA1 antibody (ABIN372626)

Chugh, Ali, Bakochi, Bahonjic, Etholm, Ekdahl: "Alterations in Brain Inflammation, Synaptic Proteins, and Adult Hippocampal Neurogenesis during Epileptogenesis in Mice Lacking Synapsin2." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132366, (2015) (PubMed).

Marowsky, Rudolph, Fritschy, Arand: "Tonic inhibition in principal cells of the amygdala: a central role for ?3 subunit-containing GABAA receptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 25, pp. 8611-9, (2012) (PubMed).

Goffin, Ali, Rampersaud, Harkavyi, Fuchs, Whitton, Nairn, Jovanovic: "Dopamine-dependent tuning of striatal inhibitory synaptogenesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 8, pp. 2935-50, (2010) (PubMed).

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABRA1 (GABRA1 Products)

Synonyms: ECA4 antibody, EJM antibody, EJM5 antibody, zgc:153566 antibody, Gabra-1 antibody, gamma-aminobutyric acid type A receptor alpha1 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 1 antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 antibody, gamma-aminobutyric acid receptor subunit alpha-1 antibody, GABRA1 antibody, Gabra1 antibody, gabra1 antibody, LOC100653509 antibody

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six Alpha's, four Beta's and four Gamma's, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for Alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a Gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different Alpha-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003).Synonyms: GABA A receptor subunit alpha-1, GABRA-1, Gamma-aminobutyric acid receptor subunit alpha-1

Gene ID: 14394

NCBI Accession: NP_034380

UniProt: P62812