NEFM antibody (C-Term)

Catalog No. ABIN372674

Product Details anti-NEFM Antibody

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Chicken

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes the ~145k Neurofilament M protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Chicken, Mouse and Rat.

Purification: Ig Fraction

Immunogen: Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: Neurofilament M (160 kD) (NEFM Products)

Synonyms: NEF3 antibody, NF-M antibody, NFM antibody, Nef3 antibody, Nfm antibody, NF160 antibody, NF165 antibody, nef-m antibody, nef3 antibody, nefm antibody, nf-m antibody, zgc:112359 antibody, nefm-a antibody, nefm-b antibody, neurofilament medium antibody, neurofilament triplet M protein antibody, neurofilament, medium polypeptide antibody, neurofilament medium polypeptide antibody, neurofilament medium L homeolog antibody, neurofilament, medium polypeptide a antibody, neurofilament medium S homeolog antibody, NEFM antibody, Nefm antibody, TVAG_582010 antibody, TVAG_379630 antibody, nefm.L antibody, nefma antibody, nefm.S antibody

Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Ref.1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (Ref.2).Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide

Gene ID: 24588

NCBI Accession: NP_058725

UniProt: P12839

Pathways: Brown Fat Cell Differentiation