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Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pTyr1336) antibody

Details for Product No. ABIN372686, Supplier: Log in to see
Antigen
  • NMDAR2B
  • GluN2B
  • MRD6
  • NR2B
  • hNR3
  • nmdar2b
  • nr2b
  • AW490526
  • Nmdar2b
Epitope
pTyr1336
50
47
39
35
31
27
17
16
14
14
14
13
13
13
12
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Non Human Primates, Rat (Rattus)
289
285
230
15
9
8
4
4
4
4
3
2
2
1
1
Host
Rabbit
290
55
14
Clonality
Polyclonal
Conjugate
Un-conjugated
12
12
10
9
9
9
9
9
9
9
9
9
5
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
240
99
84
73
71
54
43
14
8
4
3
2
1
1
1
Supplier
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1336 of the NR2B subunit of the rat NMDA receptor.
Isotype IgG
Specificity This antibody is specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1336. Immunolabeling of the NMDAR NR2B subunit band is blocked by lambda-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name NMDAR2B (GRIN2B Antibody Abstract)
Background The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B
Gene ID 2904
NCBI Accession NP_000825
UniProt Q13224
Research Area Neurology, Neurotransmitters, Receptors
Application Notes Western blot: 1/1000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pTyr1336) antibody (ABIN372686) anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pTyr1336) antibody
 image for anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pTyr1336) antibody (ABIN372686) anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pTyr1336) antibody (Image 2)
Product cited in: Hicklin, Wu, Radcliffe et al.: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, 2011 (PubMed).

Background publications Wenthold, Prybylowski, Standley et al.: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, 2003 (PubMed).

Carroll, Zukin: "NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity." in: Trends in neurosciences, Vol. 25, Issue 11, pp. 571-7, 2002 (PubMed).

Takasu, Dalva, Zigmond et al.: "Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors." in: Science (New York, N.Y.), Vol. 295, Issue 5554, pp. 491-5, 2002 (PubMed).

Grosshans, Clayton, Coultrap et al.: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." in: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, 2001 (PubMed).

Ishii, Moriyoshi, Sugihara et al.: "Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2836-43, 1993 (PubMed).