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GRIN2B antibody (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B) (pTyr1472)

Details for Product anti-GRIN2B Antibody No. ABIN372687, Supplier: Log in to see
Antigen
  • AW490526
  • GluN2B
  • hNR3
  • MRD6
  • Nmdar2b
  • nmdar2b
  • NMDAR2B
  • NR2B
  • nr2b
Epitope
pTyr1472
52
47
39
38
32
27
17
16
14
14
14
13
13
13
12
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Zebrafish (Danio rerio)
296
294
236
14
8
7
4
4
4
4
3
2
1
1
1
Host
Rabbit
296
59
14
Clonality
Polyclonal
Conjugate
This GRIN2B antibody is un-conjugated
12
12
10
9
9
9
9
9
9
9
9
9
5
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
248
99
90
75
75
56
46
17
10
4
3
2
2
1
1
Supplier
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1472 of NMDA NR2B.
Isotype IgG
Specificity This antibody is specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either lambda-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, non-Human Primates and Zebrafish.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name NMDAR2B (GRIN2B Antibody Abstract)
Background The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a, Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001, Roche et al., 2001).Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B
Gene ID 2904
NCBI Accession NP_000825
UniProt Q13224
Research Area Neurology, Neurotransmitters, Receptors
Application Notes Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-GRIN2B antibody (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B) (pTyr1472) (ABIN372687) anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pTyr1472) antibody
Product cited in: Gladding, Sepers, Xu, Zhang, Milnerwood, Lombroso, Raymond: "Calpain and STriatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington's disease mouse model." in: Human molecular genetics, Vol. 21, Issue 17, pp. 3739-52, 2012

Xu, Kurup, Bartos, Patriarchi, Hell, Lombroso: "Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity." in: The Journal of biological chemistry, Vol. 287, Issue 25, pp. 20942-56, 2012

Background publications Itoh, Matsumoto, Inoue: "Spherical aberration correction suitable for a wavefront controller." in: Optics express, Vol. 17, Issue 16, pp. 14367-73, 2009

Wenthold, Prybylowski, Standley, Sans, Petralia: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, 2003

Alvestad, Grosshans, Coultrap, Nakazawa, Yamamoto, Browning: "Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11020-5, 2003

Carroll, Zukin: "NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity." in: Trends in neurosciences, Vol. 25, Issue 11, pp. 571-7, 2002

Clayton, Grosshans, Browning: "Aging and surface expression of hippocampal NMDA receptors." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 14367-9, 2002

Clayton, Mesches, Alvarez, Bickford, Browning: "A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 9, pp. 3628-37, 2002

Nakazawa, Komai, Tezuka, Hisatsune, Umemori, Semba, Mishina, Manabe, Yamamoto: "Characterization of Fyn-mediated tyrosine phosphorylation sites on GluR epsilon 2 (NR2B) subunit of the N-methyl-D-aspartate receptor." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 693-9, 2001

Grosshans, Clayton, Coultrap, Browning: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." in: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, 2001

Roche, Standley, McCallum, Dune Ly, Ehlers, Wenthold: "Molecular determinants of NMDA receptor internalization." in: Nature neuroscience, Vol. 4, Issue 8, pp. 794-802, 2001

Snell, Nunley, Lickteig, Browning, Tabakoff, Hoffman: "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion." in: Brain research. Molecular brain research, Vol. 40, Issue 1, pp. 71-8, 1997

Ishii, Moriyoshi, Sugihara, Sakurada, Kadotani, Yokoi, Akazawa, Shigemoto, Mizuno, Masu: "Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2836-43, 1993

Lovinger, White, Weight: "Ethanol inhibits NMDA-activated ion current in hippocampal neurons." in: Science (New York, N.Y.), Vol. 243, Issue 4899, pp. 1721-4, 1989