Periostin antibody (C-Term)

Catalog No. ABIN372700

Product Details anti-Periostin Antibody

Target: Periostin (POSTN)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Chicken, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes the ~93 kDa Periostin doublet in mouse lung extract. This antibody does not recognize the single heart form of Periostin or the lowest molecular weight form of lung periostin. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes a subset of the forms of Periostin recognized on Western blots by the Pan Periostin antibody (Cat#AP08724AF-N) and also shows a distinctive staining pattern by Immunohistochemistry (data not shown).

Cross-Reactivity (Details): Species reactivity (expected):Mammalia and Avian.
Species reactivity (tested):Human, Mouse, Rat and Chicken.

Purification: Affinity Chromatography.

Immunogen: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse Periostin which is comprised of six small alternatively-spliced exons.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/100. The antibody works well for Immunohistochemistry on paraformaldehyde-fixed sectionswith a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in 10 mMsodium citrate ( pH 6.0)/ 0.1 % Tween-20).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: Periostin (POSTN Products)

Synonyms: fa99h07 antibody, pn antibody, postn antibody, wu:fa99h07 antibody, wu:fc70f09 antibody, zgc:153873 antibody, LOC100008945 antibody, osf-2 antibody, pdlpostn antibody, periostin antibody, OSF-2 antibody, OSF2 antibody, PDLPOSTN antibody, PN antibody, RP11-412K4.1 antibody, A630052E07Rik antibody, AI747096 antibody, Osf2 antibody, PLF antibody, peri antibody, Plf antibody, periostin antibody, periostin, osteoblast specific factor b antibody, periostin, osteoblast specific factor antibody, POSTN antibody, postnb antibody, postn antibody, Postn antibody

Background: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008)Synonyms: OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN

Gene ID: 10631

NCBI Accession: NP_001129406

UniProt: Q15063