PLK1 antibody (pThr210)

Catalog No. ABIN372703

Product Details anti-PLK1 Antibody

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Binding Specificity: pThr210

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Xenopus laevis, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes the ~66k PLK protein phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by lambda-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Mouse, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat and Human.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1 (PLK1 Products)

Synonyms: PLK antibody, STPK13 antibody, plk antibody, PLK-1 antibody, Plx1 antibody, stpk13 antibody, Plk antibody, cb525 antibody, cb636 antibody, ik:tdsubc_2d1 antibody, wu:fb37g11 antibody, wu:fb76g03 antibody, xx:tdsubc_2d1 antibody, polo like kinase 1 antibody, polo like kinase 1 S homeolog antibody, polo-like kinase 1 antibody, polo-like kinase 1 (Drosophila) antibody, Serine/threonine-protein kinase plk-1 antibody, PLK1 antibody, plk1.S antibody, plk1 antibody, Plk1 antibody, plk-1 antibody

Background: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK's is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1

Gene ID: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cell Division Cycle, M Phase