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Tryptophan Hydroxylase 1 antibody (pSer58)
TPH1
Reactivity: Human, Mouse, Rat, Rabbit, Cow, Chicken, Zebrafish (Danio rerio), Xenopus laevis
WB, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-Tryptophan Hydroxylase 1 Antibody
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Target
See all Tryptophan Hydroxylase 1 (TPH1) Antibodies
Tryptophan Hydroxylase 1 (TPH1)
Binding Specificity
All epitopes for Tryptophan Hydroxylase 1 antibodies
pSer58
Reactivity
All reactivities for Tryptophan Hydroxylase 1 antibodies
Human, Mouse, Rat, Rabbit, Cow, Chicken, Zebrafish (Danio rerio), Xenopus laevis
Host
All hosts for Tryptophan Hydroxylase 1 antibodies
Rabbit
Clonality
All clonalities for Tryptophan Hydroxylase 1 antibodies
Polyclonal
Conjugate
All conjugates for Tryptophan Hydroxylase 1 antibodies
This Tryptophan Hydroxylase 1 antibody is un-conjugated
Application
All applications for Tryptophan Hydroxylase 1 antibodies
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
Specific for the ~53k Tryptophan Hydroxylase protein phosphorylated at Ser58.
Cross-Reactivity (Details)
Species reactivity (expected):Bovine, Canine, Human, Mouse, Rat, Xenopus, Zebrafish and non-Human Primates. Species reactivity (tested):Rabbit.
Purification
Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of rat Tryptophan Hydroxylase 1 (TPH1).
Isotype
IgG
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Discover our top product TPH1 Primary Antibody
Alternatives
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Application Details
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Application Notes
Western blotting: 1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
Target Details for Tryptophan Hydroxylase 1
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Target
Tryptophan Hydroxylase 1 (TPH1)
Alternative Name
Tryptophan 5-Hydroxylase 1 (TPH1) (TPH1 Products )
Synonyms
TPRH antibody, TRPH antibody, tph antibody, tph1 antibody, tphD1 antibody, tph2 antibody, tph1l antibody, tphD2 antibody, Tph antibody, TPH antibody, SERGEF antibody, LOC100229598 antibody, tryptophan hydroxylase 1 antibody, tryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a antibody, TryPtophan Hydroxylase antibody, tryptophan hydroxylase 1b antibody, tryptophan hydroxylase 1 L homeolog antibody, tryptophan 5-hydroxylase 1 antibody, TPH1 antibody, tph1a antibody, tph-1 antibody, tph1b antibody, Tph1 antibody, tph1.L antibody, LOAG_02149 antibody, Tsp_05203 antibody
Background
Tryptophan Hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1
Gene ID
100009100
NCBI Accession
NP_001075741
UniProt
P17290
Pathways
Feeding Behaviour
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