You are viewing an incomplete version of our website. Please click to reload the website as full version.

UCHL1 antibody (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase))

Details for Product anti-UCHL1 Antibody No. ABIN372739, Supplier: Log in to see
Antigen
  • AW822034
  • C88048
  • cb358
  • gad
  • MGC132191
  • park5
  • PARK5
  • PGP9.5
  • PGP 9.5
  • pgp9.5
  • PGP95
  • R75593
  • uch-l1
  • UCH-L1
  • Uch-L1
  • UCHL-1
  • UCHL1
  • uchl1
  • wu:fc55h08
Reactivity
Cow (Bovine), Human, Mammalian, Mouse (Murine), Rat (Rattus)
487
239
219
110
107
68
56
47
47
47
8
7
3
2
1
Host
Chicken
257
201
34
16
6
4
Clonality
Polyclonal
Conjugate
This UCHL1 antibody is un-conjugated
19
18
16
14
10
10
9
9
9
9
9
7
7
7
7
7
7
7
7
3
2
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
413
228
176
126
103
69
64
13
12
9
6
5
3
3
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Recombinant full length human UCHL1 purified from E. coli.
Isotype Ig
Specificity Specific for the the ~24 kDa UCHL1 protein.
Cross-Reactivity (Details) Species reactivity (expected):Mamalia.
Species reactivity (tested):Bovine, Human, Mouse and Rat.
Purification Ig Fraction
Alternative Name UCHL1 / PGP9.5 (UCHL1 Antibody Abstract)
Background Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.Synonyms: Neuron cytoplasmic protein 9.5, PGP 9.5, UCH-L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1
Gene ID 7345
NCBI Accession NP_004172
UniProt P09936
Research Area Tags/Labels, Cell/Tissue Markers
Application Notes Western blotting: 1/2,500. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-UCHL1 antibody (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase)) (ABIN372739) anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody
 image for anti-UCHL1 antibody (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase)) (ABIN372739) anti-Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody (Image 2)
Background publications Liu, Fallon, Lashuel, Liu, Lansbury: "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility." in: Cell, Vol. 111, Issue 2, pp. 209-18, 2002 (PubMed).

Kurihara, Kikuchi, Wada, Tilghman: "Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia." in: Human molecular genetics, Vol. 10, Issue 18, pp. 1963-70, 2001 (PubMed).

Wilkinson, Lee, Deshpande, Duerksen-Hughes, Boss, Pohl: "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase." in: Science (New York, N.Y.), Vol. 246, Issue 4930, pp. 670-3, 1989 (PubMed).

Doran, Jackson, Kynoch, Thompson: "Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis." in: Journal of neurochemistry, Vol. 40, Issue 6, pp. 1542-7, 1983 (PubMed).