ELISA: 1/128000. Western Blot: 0.03-0.1 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5-5.0 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris Saline Buffer, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
s100a4 (S100A4)
(S100 Calcium Binding Protein A4 (S100A4))
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100A4 has been implicated in motility, invasion and tubulin polymerization. It is also involved in tumor metastasis.Synonyms: CAPL, Calvasculin, FSP-1, FSP1, Fibroblast marker, Fibroblast specific protein-1, MTS1, Metastasin, Placental Calcium-Binding Protein, S100 Calcium-Binding Protein A4, S100-A4