HPSE antibody

Catalog No. ABIN372789

Product Details anti-HPSE Antibody

Target: HPSE (Heparanase (HPSE))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes the 65 kDa precursor as well as the 55 kDa and 5 kDa subunits of Human or Mouse Heparanase.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Human 50kD-8kD Heparanase Heterodimer.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This HPSE antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody Rabbit IgG, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.001 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for HPSE

Target: HPSE (Heparanase (HPSE))

Alternative Name: Heparanase / HPSE (HPSE Products)

Synonyms: im:7144134 antibody, hpa antibody, hpa1 antibody, hpr1 antibody, hpse1 antibody, hse1 antibody, HPSE antibody, HPA antibody, HPA1 antibody, HPR1 antibody, HPSE1 antibody, HSE1 antibody, Hpa antibody, Hpr1 antibody, Hep antibody, heparanase antibody, HPSE antibody, hpse antibody, Hpse antibody

Background: Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.Synonyms: Endo-Glucoronidase, HEP, HPA, HPA1, HPR1, HPSE1, HSE1, Heparanase-1

Gene ID: 10855

NCBI Accession: NP_001092010

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process