CAMK2A antibody (pThr286)

Catalog No. ABIN372889

Product Details anti-CAMK2A Antibody

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Binding Specificity: pThr286

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes Calcium/Calmodulin-dependent Protein Kinase Type II (CAMK2). Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse, Xenopus.
Species reactivity (tested):Rat.

Purification: Immunoaffinity Chromatography

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in Rat brain CaM Kinase II.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 1/200. Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CAMK2A (CAMK2A Products)

Synonyms: camk2 antibody, camk2b antibody, zgc:112538 antibody, zgc:123320 antibody, CAMKA antibody, CaMKII antibody, R74975 antibody, mKIAA0968 antibody, PK2CDD antibody, PKCCD antibody, calcium/calmodulin dependent protein kinase II alpha antibody, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antibody, calcium/calmodulin-dependent protein kinase II alpha antibody, CAMK2A antibody, camk2a.S antibody, camk2a antibody, Camk2a antibody

Background: CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/Calmodulin-dependent Protein Kinase Type II, KIAA0968

Gene ID: 815

NCBI Accession: NP_741960

UniProt: Q9UQM7

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction