Collagen IV antibody

Catalog No. ABIN372918

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Cow, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts with most Mammalian Type IV Collagens and has negligible cross-reactivity with type I, II, III, V, or VI Collagens.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Mammal.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Collagen Type IV from Human and Bovine placenta.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 with 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to opening. This product is stable at 2-8 °C as an undiluted liquid. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at-20 °C or below. Dilute only prior to immediate use.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody

Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.Synonyms: COL4A1

Gene ID: 1282

NCBI Accession: NP_001836

UniProt: P02462