Cullin 1 (CUL1) antibody
| Antigen |
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| Synonyms |
LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (11), ELISA (6), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN373346 |
| Quantity |
0.1 ml (0,2 mg/ml) (Variants) |
| Price |
165.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Cullin-1, CUL-1, CUL1
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Alternative name
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Cullin 1
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Gene ID
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8454
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Swiss-Prot
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Q13616
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Immunogen
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A synthetic peptide derived from the C-terminus of human cullin-1.
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Cross-Reactivity
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Human, Mouse (Murine)
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Description
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Cullin-1 is a member of the family of human cullin genes (cullin-1, 2, 3, 4a, 4b, and 5)homologous to the s. cerevisiae cdc53 gene. Cullin-1 forms a complex with human p19skp1and F box protein p45skp2, both of which, together with cdc53, as three subunits, form anubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. Thecullin-1/p19skp1/p45skp2 complex is thought to play a role in the ubiquitination of G1regulatory proteins.
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Specificity
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This antibody reacts to Cullin-1. Species: Human, Mouse. Others not tested.
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Application Notes
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Western Blotting: 1-2µg/ml for 2hrs at RT. Immunoprecipitation: 2µg/mg protein lysateImmunohistochemistry on paraffin sections: 1/50 for 10 min at RT. Positive Control: Raji cells. TonsilOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0,2 mg/ml
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Purification
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Purified
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Buffer
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10mM PBS, pH 7.4 with BSA and Sodium Azide
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Storage
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Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
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Research Area
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Apoptosis/Necrosis, Proteasome
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Restrictions
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For Research Use only
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Alternatives for antigen "Cullin 1 (CUL1)", type "Antibodies"
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Hosts
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Rabbit (33)
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Reactivities
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Human (30), Mouse (Murine) (24), Rat (Rattus) (18), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1), Pig (Porcine) (1)
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Applications
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Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (11), ELISA (6), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2), C-Term AA 766-776 (1)
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