Occludin (OCLN) antibody

Antigen: Occludin (OCLN)

Synonyms: Ocl, AI503564, OCLN, ocln, oclnb, tpmt, DKFZp469D234

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN373711

Additional Information

Characteristics: Synonyms: OCLN

Alternative name: Occludin

Gene ID: 4950

Swiss-Prot: Q16625

Immunogen: Synthetic peptide corresponding to C-terminal of Human OccludinRemarks: Epitope: C-terminus

Cross-Reactivity: Human

Description: Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging upto approximately 82 kDa. This phosphorylation is thought to be involved in regulating boththe localization and the function of Occludin. Polyunsaturated fatty acids are known toup-regulate Occludin expression, increasing the transendothelial cell resistance andreducing the cellular permeability to large molecules. The level of Occludin varies greatlydepending on tissue, in brain tissue, Occludin is highly and continuously expressed atcell-cell contact sites, whereas non-neural tissues show lower expression anddiscontinuous distribution.

Specificity: This antibody recognizes Occludin. Cellular Localization: Membrane. Species Reactivity: Tested: Human. Add. Information: Molecular Weight: 65 kDa

Application Details

Application Notes: Western blotting (1/50 for 1h at RT). Immunohistochemistry on Paraffin Sections: 1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer,pH 6.0 for 10 min followed by cooling at RT for 20 min. Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Positive Control: Lung, A431 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: 10mM PBS, pH 7.4 with BSA and 0.09% Sodium Azide.

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only