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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody

Antigen

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)
Synonyms SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70
Epitope
Alternatives

C-Term
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Catalog no. ABIN374056
Quantity 0.1 ml  (0.2 mg/ml)  (Variants)
Price 195.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase
Alternative name ZAP70
Gene ID 7535
Swiss-Prot P43403
Immunogen Synthetic peptide corresponding to C-terminal of human ZAP-70
Cross-Reactivity Human
Description Mutations in ZAP-70 gene results in a form of Severe Combined ImmunodeficiencySyndrome (SCID) in humans. ZAP-70 expression also defines a subset of ChronicLymphocytic Leukaemia (CLL) in patients with unmutated Ig gene and poor clinical course.
Specificity This antibody reacts to ZAP-70 at C-term. Species: Human. Others not tested. Add. Information: Molecular weight: 70kDaCellular localisation: Cytoplasmic

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Western Blot. Immunohistochemistry on paraffin sections: 1/100 for 30min at RT, staining offormalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20 min. Positive control: Jurkat cells. Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
Purification Purified
Buffer 10 mM PBS, pH 7.4 with BSA and Sodium Azide
Storage Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
Hosts Rabbit (99), Mouse (42)
Reactivities Human (136), Mouse (Murine) (66), Rat (Rattus) (60), Cow (Bovine) (18), Pig (Porcine) (18), Dog (Canine) (17), Horse (Equine) (16), Rabbit (16), Chicken (15), Guinea Pig (15)
Applications Western Blotting (WB) (78), Flow Cytometry (FACS) (37), Immunohistochemistry (IHC) (33), Immunofluorescence (IF) (32), ELISA (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Enzyme Immunoassay (EIA) (12), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (6), FITC (6), PE (3), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
Epitopes pTyr319 (10), pTyr493 (8), Ab-319 (5), Ab-493 (5), C-Term (4), Center (3), Ab-292 (1), Ab-315 (1), pTyr292 (1), pTyr315 (1), pTyr492 (1)