AFT7 antibody (C-Term)

Catalog No. ABIN374136

Product Details anti-AFT7 Antibody

Target: AFT7 (Activating Transcription Factor 7 (AFT7))

Binding Specificity: C-Term

Reactivity: Human, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This AFT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-HVIMTPQSQS AGR

Specificity: This antibody detects ATF7 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_001123531.1, NP_006847.1, NP_001123532.1 Genename: ATF7

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 35 kDa band seen in HeLa cell lysates. Please note theband size is in agreement with previous experimental data (J Biol Chem 2001 Apr27,276(17): 13718-26). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Testis shows staining ofselect spermatocytes in Human Testis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for AFT7

Target: AFT7 (Activating Transcription Factor 7 (AFT7))

Alternative Name: ATF7 (AFT7 Products)

Synonyms: ATFA antibody, 1110012F10Rik antibody, 9430065F09Rik antibody, AI549878 antibody, C130020M04Rik antibody, ATF7 antibody, fa97a09 antibody, fc25b12 antibody, wu:fa97a09 antibody, wu:fc25b12 antibody, atfa antibody, activating transcription factor 7 antibody, activating transcription factor 7b antibody, ATF7 antibody, Atf7 antibody, atf7b antibody, atf7 antibody

Background: The cyclic AMP-dependent transcription factor ATF7 is a member of the bZIP family of proteins and is known to bind to the cAMP response element (CRE) as a homodimer.Synonyms: ATF-7, ATF-A, ATFA, Activating transcription factor 7, Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7

Gene ID: 11016

NCBI Accession: NP_001123531

UniProt: P17544