ALS2 antibody (C-Term)

Catalog No. ABIN374168

Product Details anti-ALS2 Antibody

Target: ALS2 (Amyotrophic Lateral Sclerosis 2 (Juvenile) (ALS2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ALS2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: LKACYYQIQR EKLN

Specificity: This antibody detects Alsin / ALS2 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_065970.2 Genename: ALS2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Cortex shows pixulatecytoplasm staining in some neuronal cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ALS2

Target: ALS2 (Amyotrophic Lateral Sclerosis 2 (Juvenile) (ALS2))

Alternative Name: Alsin / ALS2 (ALS2 Products)

Synonyms: als2 antibody, si:dkey-33m14.1 antibody, ALS2CR6 antibody, ALSJ antibody, IAHSP antibody, PLSJ antibody, 3222402C23Rik antibody, 9430073A21Rik antibody, Als2cr6 antibody, Alsin antibody, mKIAA1563 antibody, amyotrophic lateral sclerosis 2a (juvenile) antibody, alsin antibody, ALS2, alsin Rho guanine nucleotide exchange factor antibody, amyotrophic lateral sclerosis 2 (juvenile) antibody, als2a antibody, CpipJ_CPIJ008258 antibody, CpipJ_CPIJ008260 antibody, ALS2 antibody, Als2 antibody

Background: ALS2 specifically binds to, and functions as a guanine nucleotide exchange factor (GEF) for the small GTPase RAB5. ALS2 contains an ATS1/RCC1-like domain, a RhoGEF domain, and a vacuolar protein sorting 9 (VPS9) domain, all of which are guanine-nucleotide exchange factors that activate members of the Ras superfamily of GTPases. The protein localizes with RAB5 on early endosomal compartments, and functions as a modulator for endosomal dynamics. Loss of ALS2 function might underlie neuronal dysfunction and degeneration in a number of motor neuron diseases.Synonyms: ALS2CR6, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 6 protein, Amyotrophic lateral sclerosis protein 2, KIAA1563

Gene ID: 57679

NCBI Accession: NP_065970

UniProt: Q96Q42

Pathways: Skeletal Muscle Fiber Development