Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibody

Details for Product No. ABIN374183
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms DKK-4, Dkk-4, RGD1563172
Epitope
C-Term
(9), (4), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(24), (10), (2)
Host
Goat
(25), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1)
Application
Western Blotting (WB)
(31), (13), (11), (5), (3), (3), (2), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN374183
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide from C Terminus of the protein sequence according to NP_055235.1 Genename: DKK4
Sequence RQHARLRVCQKIEKL
Specificity This antibody detects Dickkopf-4 at C-term.
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name Dickkopf-4 (DKK4)
Background DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors. DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.
Alternate names: Dickkopf-related protein 4, Dkk-4
Gene ID 27121
NCBI Accession NP_055235.1
UniProt Q9UBT3
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 µg/ml. Approx 30-35 kDa band seen in Jurkat lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Expiry Date 12 months
Supplier Images
anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibody anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (C-Term) antibody
Background publications Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).

Hosts (25), (5), (2)
Reactivities (24), (10), (2)
Applications (31), (13), (11), (5), (3), (3), (2), (1), (1)
Conjugates (1), (1)
Epitopes (9), (4), (2), (2), (2), (1), (1), (1)
back to top