KLF1 antibody (N-Term)

Catalog No. ABIN374197

Product Details anti-KLF1 Antibody

Target: KLF1 (Kruppel-Like Factor 1 (erythroid) (KLF1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF1 antibody is un-conjugated

Application: Chromatin Immunoprecipitation (ChIP)

Sequence: ATAETALPSI STLT-C

Specificity: This antibody detects Krueppel-like factor 1 (KLF1) at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the N-Terminus of the protein sequence according to NP_006554.1 Genename: KLF1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. ABIN374197 has been successfully used in ChiP, see Hou et al. , PNAS, 2008. ChIP: Please see: Hou C. et al. CTCF-dependent enhancer-blocking by alternative chromatinloop formation. Proc Natl Acad Sci U S A.2008 Dec 23,105(51): 20398-403. Epub 2008 Dec12.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KLF1

Target: KLF1 (Kruppel-Like Factor 1 (erythroid) (KLF1))

Alternative Name: Krueppel-Like Factor 1 (KLF1) (KLF1 Products)

Synonyms: KLF1 antibody, EKLF antibody, HBFQTL6 antibody, INLU antibody, Eklf antibody, Nan antibody, Kruppel-like factor 1 (erythroid) antibody, Kruppel like factor 1 antibody, KLF-4 antibody, KLF1 antibody, Klf1 antibody

Background: EKLF / KLF1 is an essential transcriptional activator that directs high-level expression of the adult beta-globin promoter by binding to its CACCC element. Probably serves as a general switch factor for erythroid development. Human EKLF is expressed in bone marrow and erythroleukemic cells lines but not in myeloid or lymphoid cell lines.Synonyms: EKLF, Erythroid krueppel-like transcription factor, Erythroid transcription factor, KLF-1, KLF1, Krueppel-like factor 1

Gene ID: 10661

NCBI Accession: NP_006554

UniProt: Q13351