Peptide ELISA: Limit dilution 1: 32000. ABIN374197 has been successfully used in ChiP, see Hou et al. , PNAS, 2008. ChIP: Please see: Hou C. et al. CTCF-dependent enhancer-blocking by alternative chromatinloop formation. Proc Natl Acad Sci U S A.2008 Dec 23,105(51): 20398-403. Epub 2008 Dec12. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
EKLF / KLF1 is an essential transcriptional activator that directs high-level expression of the adult beta-globin promoter by binding to its CACCC element. Probably serves as a general switch factor for erythroid development. Human EKLF is expressed in bone marrow and erythroleukemic cells lines but not in myeloid or lymphoid cell lines.Synonyms: EKLF, Erythroid krueppel-like transcription factor, Erythroid transcription factor, KLF-1, KLF1, Krueppel-like factor 1