KLF15 antibody (N-Term)

Catalog No. ABIN374226

Product Details anti-KLF15 Antibody

Target: KLF15 (Kruppel-Like Factor 15 (KLF15))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This KLF15 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VDHLLPVDEN FSS-C

Specificity: This antibody detects KLF15 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Pig.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_054798.1 Genename: KLF15

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1.0 μg/mL. Approx 48 kDa band observed in lysates of hepatoblastomacell line HepG2 (calculated MW of 44.8 kDa according to NP_054798.1). Recommended(Data obtained with a previous EB05463 batch). Identical WB results were obtained with

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for KLF15

Target: KLF15 (Kruppel-Like Factor 15 (KLF15))

Alternative Name: KLF15 (KLF15 Products)

Synonyms: KKLF antibody, 1810013I09Rik antibody, AV048136 antibody, AW494632 antibody, CKLF antibody, Kruppel like factor 15 antibody, Kruppel-like factor 15 S homeolog antibody, Kruppel-like factor 15 antibody, KLF15 antibody, klf15.S antibody, Klf15 antibody

Background: The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84 % identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.Synonyms: CKLF, Cardiovascular Krueppel-like factor, KKLF, KLF15, Kidney-enriched krueppel-like factor, Krueppel-like factor 15

Gene ID: 28999

NCBI Accession: NP_054798

UniProt: Q9UIH9