ELF3 antibody (C-Term)

Catalog No. ABIN374234

Product Details anti-ELF3 Antibody

Target: ELF3 (E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ELF3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects ELF3 / ESE-1 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-SGWKEEEVLQSRN, from the C Terminus of the protein sequence Genename: ELF3

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 2 - 4 μg/mL. Approx 48-50 kDa band seen in NCI-H460 lysate (predicted MW40 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ELF3

Target: ELF3 (E74-Like Factor 3 (Ets Domain Transcription Factor, Epithelial-Specific) (ELF3))

Alternative Name: ELF3 / ESE-1 (ELF3 Products)

Synonyms: ert antibody, esx antibody, epr-1 antibody, ese-1 antibody, ESE-1 antibody, ESX antibody, jen antibody, EPR-1 antibody, ERT antibody, E74 like ETS transcription factor 3 antibody, E74-like factor 3 antibody, elf3 antibody, Elf3 antibody, ELF3 antibody

Background: ESE1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE1 is a critical regulator of epithelial cell differentiation.Synonyms: E74-like factor 3, ERT, ESX, ETS-related transcription factor Elf-3, Elf-3, Epithelial-restricted with serine box, Epithelium-restricted Ets protein ESX, Epithelium-specific Ets transcription factor 1, JEN

Gene ID: 1999

NCBI Accession: NP_004424

UniProt: P78545