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Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody

Details for Product No. ABIN374291, Supplier: Log in to see
Antigen
  • UBC9
  • T13J8.70
  • T13J8_70
  • UBIQUITIN-PROTEIN LIGASE
  • ubiquitin conjugating enzyme 9
  • C358B7.1
  • P18
  • 5830467E05Rik
  • F830028O17Rik
  • Ubce2i
  • Ubce9
  • UbcE2A
  • ubc9
  • ube2ia
  • zUbc9
  • ubce9
Epitope
N-Term
35
5
3
2
2
2
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
106
32
25
8
6
3
3
3
2
2
1
Host
Goat
70
20
17
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
4
4
4
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
96
60
50
9
9
9
6
5
4
2
1
1
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Immunogen Peptide from the N Terminus of the protein sequence according to NP_003336.1, NP_919235.1, NP_919236.1, NP_919237.1 Genename: UBE2I
Sequence SGIALSRLAQ ERK-C
Specificity This antibody detects UBC9 / UBE2I at N-term. It is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Cross-Reactivity (Details) Species reactivity (expected):Canien (Dog).
Species reactivity (tested):Human, Mouse, Rat.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name UBC9 / UBE2I (UBE2I Antibody Abstract)
Background Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I
Gene ID 7329
NCBI Accession NP_003336
UniProt P63279
Application Notes Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (ABIN374291) anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody
 image for anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (ABIN374291) anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (Image 2)
Background publications Subramaniam, Mealer, Sixt et al.: "Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20428-32, 2010 (PubMed).

Ihara, Stein, Schultz: "UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes." in: Biology of reproduction, Vol. 79, Issue 5, pp. 906-13, 2008 (PubMed).

Wang, Qiu, Seufert et al.: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, 1996 (PubMed).