ARIH1 antibody (C-Term)

Catalog No. ABIN374297

Product Details anti-ARIH1 Antibody

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ARIH1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-HEGYEKDLWE YIED

Specificity: This antibody detects ARIH1 / UBCH7BP at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005735.2 Genename: ARIH1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 65 kDa band seen in U937 lysate [Predicted MW of approx.65 kDa according toNP_005735].
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ARIH1

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Alternative Name: ARIH1 / UBCH7BP (ARIH1 Products)

Synonyms: ARIH1 antibody, ARI-1 antibody, ari antibody, ariadne antibody, hari antibody, hhari antibody, ubch7bp antibody, ARI antibody, HARI antibody, HHARI antibody, UBCH7BP antibody, AU021774 antibody, Ari antibody, Ari1 antibody, Hari antibody, Hhari antibody, Ubch7bp antibody, Uip77 antibody, wu:fa18d01 antibody, zgc:66364 antibody, ariadne RBR E3 ubiquitin protein ligase 1 antibody, ariadne RBR E3 ubiquitin protein ligase 1 L homeolog antibody, ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) antibody, Arih1 antibody, ARIH1 antibody, arih1 antibody, arih1.L antibody

Background: ARIH1 might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.Synonyms: ARI, H7-AP2, HHARI, HUSSY-27, MOP6, Monocyte protein 6, Protein ariadne-1 homolog, UbcH7-binding protein, UbcM4-interacting protein, Ubiquitin-conjugating enzyme E2-binding protein 1

Gene ID: 25820

NCBI Accession: NP_005735

UniProt: Q9Y4X5