RNF2 antibody (C-Term)

Catalog No. ABIN374300

Product Details anti-RNF2 Antibody

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-PMELYYAPTK EHK

Specificity: This antibody detects RNF2 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Dog, Cow.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide from C-Terminus of Human RNF2. Genename: RNF2

Application Details

Application Notes: ELISA (1/4000). Western blot (0.3-1 μg/mL). Immunohistochemistry on Paraffin Sections (3.75 μg/mL). This antibody validated for use in Immunohistochemistry on a panel of 21 formalin-fixed,paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0citrate buffer. After incubation with the primary antibody, slides were incubated withbiotinylated secondary antibody, followed by alkaline phosphatase-streptavidin andchromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Alternative Name: RNF2 (RNF2 Products)

Synonyms: BAP-1 antibody, BAP1 antibody, DING antibody, HIPI3 antibody, RING1B antibody, RING2 antibody, AI326319 antibody, AI450156 antibody, AU019207 antibody, Ring1B antibody, dinG antibody, fa19h05 antibody, ring1b antibody, wu:fa19h05 antibody, wz4556 antibody, bap-1 antibody, bap1 antibody, ding antibody, hipi3 antibody, ring2 antibody, ring finger protein 2 antibody, ring finger protein 2 L homeolog antibody, RNF2 antibody, Rnf2 antibody, rnf2 antibody, rnf2.L antibody

Background: RING1B (also known as BAP1, DING, Polycomb-M33 interacting protein Ring1B, Ring finger protein 1b, Ring finger protein 2 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity.Synonyms: BAP-1, BAP1, DING, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, HIPI3, Protein DinG, RING finger protein 2, RING1B

Gene ID: 6045

NCBI Accession: NP_009143

UniProt: Q99496