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CARD11 antibody (Caspase Recruitment Domain Family, Member 11) (C-Term)

Details for Product anti-CARD11 Antibody No. ABIN374314, Supplier: Log in to see
Antigen
  • 0610008L17Rik
  • 2410011D02Rik
  • BENTA
  • BIMP3
  • CARD11
  • CARMA1
  • LOC100232755
  • PPBL
Alternatives
anti-Human CARD11 antibody for ELISA
Epitope
C-Term
27
7
6
4
3
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine)
66
27
4
3
3
1
1
Host
Goat
50
18
1
1
Clonality
Polyclonal
Conjugate
This CARD11 antibody is un-conjugated
1
1
1
1
1
1
Application
Western Blotting (WB)
64
38
33
16
6
5
4
3
2
1
Supplier
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Immunogen Peptide from the C Terminus of the protein sequence according to NP_115791.3 Genename: CARD11
Sequence C-QRKTIWVDED QL
Specificity This antibody detects CARD11 / CARMA1 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Dog, CowSpecies reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name CARD11 / CARMA1 (CARD11 Antibody Abstract)
Background CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.Synonyms: CARD-containing MAGUK protein 3, Caspase recruitment domain-containing protein 11
Gene ID 84433
NCBI Accession NP_115791
UniProt Q9BXL7
Research Area Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western Blot: 1-3 μg/mL. Approx 150+65 kDa bands observed in lysates of cell line Jurkat(calculated MW of 135 kDa according to NP_115791.2). A similar pattern was observed inHEK293 transiently expressing CARD11. These bands are not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-CARD11 antibody (Caspase Recruitment Domain Family, Member 11) (C-Term) (ABIN374314) anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody
 image for anti-CARD11 antibody (Caspase Recruitment Domain Family, Member 11) (C-Term) (ABIN374314) anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (Image 2)
Background publications Bertin, Wang, Guo, Jacobson, Poyet, Srinivasula, Merriam, DiStefano, Alnemri: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001