NUP50 antibody (C-Term)

Catalog No. ABIN374317

Product Details anti-NUP50 Antibody

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This NUP50 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-ELHKILLEKK DA

Specificity: This antibody detects NUP50 at C-term. It is expected to recognise both human isoforms of this protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_009103.2, NP_705931.1. Genename: NUP50

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 60 kDa band seen in Jurkat lysate (predicted MW 54 kDaaccording to NP_009103).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: NUP50 (NUP50 Products)

Synonyms: wu:fa56e03 antibody, wu:fb78a08 antibody, GB18194 antibody, npap60 antibody, npap60l antibody, Nup50 antibody, Rtp60 antibody, 1700030K07Rik antibody, AI413123 antibody, Npap60 antibody, NPAP60 antibody, NPAP60L antibody, nucleoporin 50 antibody, nuclear pore complex protein Nup50 antibody, nucleoporin 50kDa S homeolog antibody, nucleoporin 50kDa antibody, nup50 antibody, LOC410864 antibody, NUP50 antibody, nup50.S antibody, CpipJ_CPIJ012737 antibody, Nup50 antibody

Background: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa

Gene ID: 10762

NCBI Accession: NP_009103

UniProt: Q9UKX7

Pathways: Tube Formation