Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 37 kDa band observed in Human Brain lysates (calculatedMW of 37.7 kDa according to NP_149084.1, isoform delta). An additional faint 47 kDa bandconsistent with calculated MW of 40.7 kDa according to NP_149083.1 (isoform gamma) isalso observed. Immunohistochemistry: 1.0 μg/mL. In paraffin embedded Human Tonsil,stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptaeand weak in germinal centre. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.