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FOXA1 antibody (Forkhead Box A1) (C-Term)

Details for Product anti-FOXA1 Antibody No. ABIN374334, Supplier: Log in to see
Antigen
  • FoxA4a
  • XFD-1
  • fkh1
  • foxa1
  • pintallavis
  • xfkh1
  • xkfh-1
  • HNF3-alpha
  • HNF3alpha
  • Hnf3a
  • fkh2
  • tFoxA1
  • tcf3a
  • xfkh2
  • fkh
  • FOXA1
  • Ci-fkh
  • foxa3
  • Foxa1
  • fkd7
  • forkhead-7
  • hnf3a
  • HNF3A
  • TCF3A
  • HNF-3a
  • Hnf-3a
  • Tcf-3a
  • Tcf3a
  • forkhead box A4
  • forkhead box A1
  • forkhead homolog
  • Me-FKH1
  • foxa4
  • foxa1
  • fkh
  • FOXA1
  • foxa3
  • LOC100301627
  • foxa1-b
  • Foxa1
Alternatives
anti-Cow (Bovine) FOXA1 antibody for Western Blotting
Epitope
C-Term
33
23
15
12
11
10
10
9
9
7
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
345
108
44
12
7
6
5
4
4
3
2
2
1
1
Host
Goat
245
81
19
Clonality
Polyclonal
Conjugate
This FOXA1 antibody is un-conjugated
12
12
12
11
9
9
8
8
8
8
8
8
8
8
8
8
6
3
3
2
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
223
206
188
178
151
93
80
13
12
11
7
6
2
1
1
1
1
Options
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Immunogen Peptide from C-Terminus of the protein sequence according to NP_004487.2 Genename: FOXA1
Sequence C-GVYSRPVLNT S
Specificity This antibody detects FOXA1.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Pig, Cow.
Species reactivity (tested):Human, Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name FOXA1 / TCF3A (FOXA1 Antibody Abstract)
Background FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.Synonyms: Forkhead box protein A1, HNF-3-alpha, HNF-3A, HNF3A, Hepatocyte nuclear factor 3-alpha, TCF-3A, Transcription factor 3A
Molecular Weight 49.1 kDa (NP_004487.2)
Gene ID 3169
NCBI Accession NP_004487
UniProt P55317
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis
Application Notes Peptide ELISA: Limit dilution 1/16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in Human lysates of cell lineHepG2 (calculated MW of 49.0 kDa according to NP_004487.2). An additional band ofunknown identity was also consistently observed at 30 kDa. This band was successfullyblocked by incubation with the immunising peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Anonymous customers used this product successfully in ChIP with MCF7.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-FOXA1 antibody (Forkhead Box A1) (C-Term) (ABIN374334) anti-Forkhead Box A1 (FOXA1) (C-Term) antibody
 image for anti-FOXA1 antibody (Forkhead Box A1) (C-Term) (ABIN374334) anti-Forkhead Box A1 (FOXA1) (C-Term) antibody (Image 2)
Background publications McMullin, Dobi, Mutton, Orosz, Maheshwari, Shashikant, Bieberich: "A FOXA1-binding enhancer regulates Hoxb13 expression in the prostate gland." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 1, pp. 98-103, 2010 (PubMed).

Taube, Allton, Duncan, Shen, Barton: "Foxa1 functions as a pioneer transcription factor at transposable elements to activate Afp during differentiation of embryonic stem cells." in: The Journal of biological chemistry, Vol. 285, Issue 21, pp. 16135-44, 2010 (PubMed).

Hua, Kittler, White: "Genomic antagonism between retinoic acid and estrogen signaling in breast cancer." in: Cell, Vol. 137, Issue 7, pp. 1259-71, 2009 (PubMed).

Eeckhoute, Lupien, Meyer, Verzi, Shivdasani, Liu, Brown: "Cell-type selective chromatin remodeling defines the active subset of FOXA1-bound enhancers." in: Genome research, Vol. 19, Issue 3, pp. 372-80, 2009 (PubMed).

Lupien, Eeckhoute, Meyer, Wang, Zhang, Li, Carroll, Liu, Brown: "FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription." in: Cell, Vol. 132, Issue 6, pp. 958-70, 2008 (PubMed).

Eeckhoute, Carroll, Geistlinger, Torres-Arzayus, Brown: "A cell-type-specific transcriptional network required for estrogen regulation of cyclin D1 and cell cycle progression in breast cancer." in: Genes & development, Vol. 20, Issue 18, pp. 2513-26, 2006 (PubMed).

Carroll, Liu, Brodsky, Li, Meyer, Szary, Eeckhoute, Shao, Hestermann, Geistlinger, Fox, Silver, Brown: "Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1." in: Cell, Vol. 122, Issue 1, pp. 33-43, 2005 (PubMed).

Nguyen, Cho, Stratton, Barton: "Transcription factor interactions and chromatin modifications associated with p53-mediated, developmental repression of the alpha-fetoprotein gene." in: Molecular and cellular biology, Vol. 25, Issue 6, pp. 2147-57, 2005 (PubMed).