FOXL2 antibody (C-Term)

Catalog No. ABIN374339

Product Details anti-FOXL2 Antibody

Target: FOXL2 (Forkhead Box L2 (FOXL2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-DSKTGALHSR LDL

Specificity: This antibody detects FOXL2 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Cow, Rat.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_075555.1 Genename: FOXL2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-2 μg/mL. Approx 45-50 kDa band observed in Human Ovary extracts(predicted MW of 40 kDa according to NP_075555).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for FOXL2

Target: FOXL2 (Forkhead Box L2 (FOXL2))

Alternative Name: FOXL2 (FOXL2 Products)

Synonyms: BPES antibody, BPES1 antibody, PFRK antibody, PINTO antibody, POF3 antibody, AU045128 antibody, Pfrk antibody, FOXL2 antibody, zgc:136549 antibody, foxl2 antibody, FoxL2 antibody, foxL2 antibody, forkhead box L2 antibody, forkhead box L2a antibody, forkhead box L2 S homeolog antibody, forkhead transcription factor L2 antibody, foxl2 protein antibody, fork-head box L2 transcription factor antibody, FOXL2 antibody, Foxl2 antibody, foxl2a antibody, foxl2 antibody, foxl2.S antibody, LOC751846 antibody, foxL2 antibody

Background: There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles, in adult humans, it appeared predominantly in the ovary.Synonyms: Forkhead box protein L2

Gene ID: 668

NCBI Accession: NP_075555

UniProt: P58012

Pathways: Nuclear Hormone Receptor Binding, Positive Regulation of Endopeptidase Activity