KDM2B antibody (C-Term)

Catalog No. ABIN374346

Product Details anti-KDM2B Antibody

Target: KDM2B (Lysine (K)-Specific Demethylase 2B (KDM2B))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Dog, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This KDM2B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-FGQVEEKLLQ KLS

Specificity: This antibody detects FBXL10 / PCCX2 at C-term. It is expected to recognize isoform a (NP_115979.3) only.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_115979.3 Genename: KDM2B

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Customer's experiments gave bands at approx 170+90 kDa inlysates of cell line HeLa (calculated MW of 150 kDa according to NP_115979.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for KDM2B

Target: KDM2B (Lysine (K)-Specific Demethylase 2B (KDM2B))

Alternative Name: KDM2B / FBXL10 (KDM2B Products)

Synonyms: CXXC2 antibody, FBXL10 antibody, Fbl10 antibody, JHDM1B antibody, PCCX2 antibody, fbxl10 antibody, wu:fd13a09 antibody, zgc:85787 antibody, KDM2B antibody, Fbxl10 antibody, Cxxc2 antibody, E430001G17 antibody, Jhdm1b antibody, mKIAA3014 antibody, jhdm1b antibody, F6P23.18 antibody, F6P23_18 antibody, lysine demethylase 2B antibody, lysine (K)-specific demethylase 2Ba antibody, microRNA 7107 antibody, lysine (K)-specific demethylase 2B antibody, lysine (K)-specific demethylase 2B L homeolog antibody, F-box/RNI-like superfamily protein antibody, KDM2B antibody, kdm2ba antibody, Kdm2b antibody, MIR7107 antibody, kdm2b.L antibody, AT2G17020 antibody

Background: KDM2B is a histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylted H3 'Lys-36'. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.Synonyms: CXXC-type zinc finger protein 2, CXXC2, F-box and leucine-rich repeat protein 10, F-box protein FBL10, F-box/LRR-repeat protein 10, FBL10, JEMMA, JHDM1B, JmjC domain-containing histone demethylation protein 1B, Jumonji domain-containing EMSY-interactor methyltransferase motif protein, Lysine-specific demethylase 2B, PCCX2, Protein-containing CXXC domain 2, [Histone-H3]-lysine-36 demethylase 1B

Gene ID: 84678

NCBI Accession: NP_115979

UniProt: Q8NHM5

Pathways: Tube Formation, Warburg Effect