Pleckstrin antibody (C-Term)

Catalog No. ABIN374378

Product Details anti-Pleckstrin Antibody

Target: Pleckstrin (PLEK)

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Pleckstrin antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-EWIKAIQMAS RTGK

Specificity: This antibody detects Pleckstrin at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002655.2 Genename: PLEK

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.05-1 μg/mL. Approx 45 kDa band observed in Human Heart and PBMClysates (predicted MW of 45 kDa according to NP_002655).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Pleckstrin

Target: Pleckstrin (PLEK)

Alternative Name: Pleckstrin / PLEK (PLEK Products)

Synonyms: MGC69065 antibody, PLEK antibody, P47 antibody, 2010300B13Rik antibody, pleckstrin L homeolog antibody, pleckstrin antibody, plek.L antibody, PLEK antibody, Plek antibody

Background: Synonyms: Platelet p47 protein

Gene ID: 5341

NCBI Accession: NP_002655

UniProt: P08567

Pathways: Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process