PARD6A antibody (C-Term)

Catalog No. ABIN374388

Product Details anti-PARD6A Antibody

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PARD6A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-GSRIRGDGSG FSL

Specificity: This antibody detects PARD6A at C-term. It is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Syntehtic Peptide from C-Terminus of Human PARD6A Genename: PARD6A

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 8000. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Pancreas, shows strongcytoplasmic staining in rare cells of intralobular ducts. Recommended concentration,10 μg/mL. Also tested in paraffin embedded Human Heart where it shows speckled stainingon myocardial fibres in transverse section.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PARD6A

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Alternative Name: PARD6A (PARD6A Products)

Synonyms: PARD6A antibody, 0710008C04Rik antibody, 2610010A15Rik antibody, PAR6alpha antibody, Par-6 antibody, Par6 antibody, Par6c antibody, TAX40 antibody, Tip-40 antibody, Par-6a antibody, Par6a antibody, PAR-6A antibody, PAR6 antibody, PAR6C antibody, TIP-40 antibody, par-6 partitioning defective 6 homolog alpha (C. elegans) antibody, par-6 family cell polarity regulator alpha antibody, Partitioning defective protein 6 antibody, PARD6A antibody, Pard6a antibody, par-6 antibody

Background: PAR6 is a cell membrane protein involved in cell polarization and asymmetrical cell division processes as part of a multi protein complex. It is thought to be involved in the invasive phenotype that is associated with metastatic carcinomas, playing a role in the epithelial to mesenchymal transition (EMT). PAR6 is also thought to be involved in the formation of epithelial tight junctions. PAR6 contains a semi Cdc42/Rac interactive binding (CRIB) domain and a PDZ (PSD95/Discs-large/ZO1) domain, and several different PAR6 isoforms have been identified.Synonyms: PAR-6, PAR-6 alpha, PAR-6A, PAR6A, PAR6C, Partitioning defective 6 homolog alpha, TIP-40, Tax interaction protein 40

Gene ID: 50855

NCBI Accession: NP_058644

UniProt: Q9NPB6

Pathways: Cell-Cell Junction Organization