GOPC antibody (C-Term)
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- Target See all GOPC Antibodies
- GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))
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Binding Specificity
- C-Term
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Reactivity
- Human, Cow, Dog
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This GOPC antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Sequence
- C-LDDLHTLYHK KSY
- Specificity
- This antibody detects PIST / FIG / GOPC at C-term. It is expected to recognize both reported isoforms.
- Cross-Reactivity (Details)
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Species reactivity (expected):Canine (Dog), Cow.
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogen
- Peptide from C-Terminus of the protein sequence according to NP_065132.1, NP_001017408.1 Genename: GOPC
- Top Product
- Discover our top product GOPC Primary Antibody
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- Application Notes
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Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.05-0.2 μg/mL. Approx.55 kDa band observed in Human Brain (FrontalCortex) lysates (calculated MW of 50.5 kDa according to NP_065132.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))
- Alternative Name
- GOPC / PIST (GOPC Products)
- Synonyms
- DKFZp459H2130 antibody, 2210402P09Rik antibody, AI844555 antibody, CAL antibody, FIG antibody, GOPC1 antibody, PIST antibody, fe38f01 antibody, wu:fe38f01 antibody, zgc:56254 antibody, zgc:65853 antibody, dJ94G16.2 antibody, cal antibody, gopc1 antibody, pist antibody, golgi associated PDZ and coiled-coil motif containing antibody, golgi-associated PDZ and coiled-coil motif containing antibody, golgi-associated PDZ and coiled-coil motif containing S homeolog antibody, GOPC antibody, Gopc antibody, gopc antibody, gopc.S antibody
- Background
- PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.Synonyms: CAL, CFTR-associated ligand, FIG, Fused in glioblastoma, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10
- Gene ID
- 57120
- NCBI Accession
- NP_065132
- UniProt
- Q9HD26
- Pathways
- Maintenance of Protein Location, Asymmetric Protein Localization
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