VPS35 antibody (C-Term)

Catalog No. ABIN374403

Product Details anti-VPS35 Antibody

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS35 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-SPESEGPIYE GLIL

Specificity: This antibody detects VPS35 / MEM3 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Syntehtic peptide from the C-Terminus of Human VPS35 (NP_060676.2). Genename: VPS35

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 90 kDa band observed in Human Brain and Testis lysates (predictedMW of 90 kDa according to NP_060676.2). Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Testis, shows positive staining inLeydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymisand of the rete testis. Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please seeScott KL et al. , Nature.2009 Jun 25,459(7250): 1085-90. , PMID: 19553991.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35 / MEM3 (VPS35 Products)

Synonyms: MEM3 antibody, PARK17 antibody, vps35 antibody, MGC81959 antibody, bZ34G2.3 antibody, si:rp71-34g2.3 antibody, wu:fa66e03 antibody, wu:fb95c08 antibody, zgc:136268 antibody, 23.m05999 antibody, Mem3 antibody, VPS35 antibody, DKFZp459E1510 antibody, CG5625 antibody, DVps35 antibody, Dmel\\CG5625 antibody, Dvps35 antibody, dvps35 antibody, AI647796 antibody, mem3 antibody, VPS35, retromer complex component antibody, VPS35 retromer complex component L homeolog antibody, VPS35 retromer complex component antibody, vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, vacuolar protein sorting-associated protein 35 antibody, vacuolar protein sorting 35 antibody, Vacuolar protein sorting 35 antibody, hypothetical protein antibody, VPS35 retromer complex component S homeolog antibody, retromer complex subunit Vps35 antibody, VPS35 antibody, vps35.L antibody, vps35 antibody, Tc00.1047053511367.170 antibody, Tb10.70.5460 antibody, BBOV_IV009740 antibody, CC1G_12185 antibody, PTRG_08682 antibody, CMU_017400 antibody, PHATR_43830 antibody, MCYG_00751 antibody, MGYG_01212 antibody, EHI_086580 antibody, EHI_002990 antibody, EHI_041950 antibody, EHI_096520 antibody, Bm1_44045 antibody, LOC100285267 antibody, Vps35 antibody, PGTG_10018 antibody, Tsp_09825 antibody, vps35.S antibody

Background: Synonyms: Maternal-embryonic 3, TCCCTA00141, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Gene ID: 55737

NCBI Accession: NP_060676

UniProt: Q96QK1