Transgelin antibody (C-Term)

Catalog No. ABIN374407

Product Details anti-Transgelin Antibody

Target: Transgelin (TAGLN)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Transgelin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-MTGYGRPRQI IS

Specificity: This antibody detects Transgelin (TAGLN) at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_003177.2. Genename: TAGLN

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.01-0.03 mg/mL. Approx 24 kDa band observed in Human Placenta Lysates(calculated MW of 22.4 kDa according to NP_003177.2 and NP_001001522.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Transgelin antibody (ABIN374407)

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Huet, Fanget, Jouannot, Meireles, Zeiske, Larochette, Darchen, Desnos: "Myrip couples the capture of secretory granules by the actin-rich cell cortex and their attachment to the plasma membrane." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 7, pp. 2564-77, (2012) (PubMed).

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Cencetti, Bernacchioni, Nincheri, Donati, Bruni: "Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis." in: Molecular biology of the cell, Vol. 21, Issue 6, pp. 1111-24, (2010) (PubMed).

Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).

Nincheri, Luciani, Squecco, Donati, Bernacchioni, Borgognoni, Luciani, Benvenuti, Francini, Bruni: "Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 10, pp. 1741-54, (2009) (PubMed).

Donoghue, Doran, Wynne, Pedersen, Dunn, Ohlendieck: "Proteomic profiling of chronic low-frequency stimulated fast muscle." in: Proteomics, Vol. 7, Issue 18, pp. 3417-30, (2007) (PubMed).

Saijou, Itoh, Kim, Iemura, Natsume, Miyajima: "Nucleocytoplasmic shuttling of the zinc finger protein EZI Is mediated by importin-7-dependent nuclear import and CRM1-independent export mechanisms." in: The Journal of biological chemistry, Vol. 282, Issue 44, pp. 32327-37, (2007) (PubMed).

Nakamura, Moore, Negishi, Sueyoshi: "Nuclear pregnane X receptor cross-talk with FoxA2 to mediate drug-induced regulation of lipid metabolism in fasting mouse liver." in: The Journal of biological chemistry, Vol. 282, Issue 13, pp. 9768-76, (2007) (PubMed).

Tamimi, Skarie, Footz, Berry, Link, Walter: "FGF19 is a target for FOXC1 regulation in ciliary body-derived cells." in: Human molecular genetics, Vol. 15, Issue 21, pp. 3229-40, (2006) (PubMed).

Target Details for Transgelin

Target: Transgelin (TAGLN)

Abstract: TAGLN Products

Synonyms: MGC68550 antibody, sm22 antibody, smcc antibody, tagln1 antibody, ws3-10 antibody, MGC107855 antibody, SM22 antibody, SMCC antibody, TAGLN1 antibody, WS3-10 antibody, Sm22 antibody, Sm22a antibody, Ws310 antibody, SM22A antibody, zgc:153186 antibody, transgelin L homeolog antibody, transgelin antibody, tagln.L antibody, tagln antibody, LOC732906 antibody, TAGLN antibody, tagl antibody, LOC100009332 antibody, Tagln antibody

Background: TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.Synonyms: 22 kDa actin-binding protein, SM22, Smooth muscle protein 22-alpha, WS3-10

Gene ID: 6876

NCBI Accession: NP_003177

UniProt: Q01995