Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

NOXO1 antibody (C-Term)

NOXO1 Reactivity: Human IHC (p) Host: Goat Polyclonal unconjugated
Catalog No. ABIN374411
  • Target See all NOXO1 Antibodies
    NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))
    Binding Specificity
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 28
    • 1
    • 1
    • 1
    Human
    Host
    • 26
    • 1
    • 1
    Goat
    Clonality
    • 28
    Polyclonal
    Conjugate
    • 15
    • 3
    • 3
    • 3
    • 2
    • 2
    This NOXO1 antibody is un-conjugated
    Application
    • 23
    • 19
    • 4
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Sequence
    RGCVDSVPHP TTEQ
    Specificity
    This antibody detects NOXO1 at C-term. It is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).
    Cross-Reactivity (Details)
    Species reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogen
    Peptide from the C Terminus of the protein sequence according to NP_653204.1, NP_751907.1, NP_751907.1. Genename: NOXO1
    Top Product
    Discover our top product NOXO1 Primary Antibody
  • Application Notes
    Peptide ELISA: Limit dilution 1: 16000. Immunohistochemistry: 10-20 μg/mL. In paraffin embedded Human Kidney Cortex showscytoplasmic stain in epithelial cells of some PCT.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Target
    NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))
    Alternative Name
    NOXO1 (NOXO1 Products)
    Synonyms
    NOXO1 antibody, cb18 antibody, sb:cb18 antibody, wu:fd09d09 antibody, noxo1 antibody, snx28 antibody, p41nox antibody, p41noxa antibody, p41noxb antibody, p41noxc antibody, sh3pxd5 antibody, P41NOX antibody, P41NOXA antibody, P41NOXB antibody, P41NOXC antibody, SH3PXD5 antibody, SNX28 antibody, 2310034C04Rik antibody, Snx28 antibody, hslt antibody, NADPH oxidase organizer 1 antibody, NOXO1 antibody, noxo1 antibody, noxo1.1 antibody, Noxo1 antibody
    Background
    NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Cotransfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5 P2, PtdIns 5 P and PtdIns 4 P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 colocalizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5
    Gene ID
    124056
    NCBI Accession
    NP_653204
    UniProt
    Q8NFA2
You are here:
Support