DDX5 antibody (C-Term)

Catalog No. ABIN374414

Product Details anti-DDX5 Antibody

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This DDX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-PMIGYPMPTG YSQ

Specificity: This antibody detects DEAD Box Protein 5 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_004387.1. Genename: DDX5

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.11-0.3 μg/mL. Approx 70 kDa band observed in human epidermoidcarcinoma A431 lysates (calculated MW of 69.0 kDa according to NP_004387). Immunofluorescence: A customer has reported successful staining at a dilution of 1: 100 onHT-1080 cells with a Rhodamine conjugated secondary.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name: DDX5 (DDX5 Products)

Synonyms: G17P1 antibody, HLR1 antibody, HUMP68 antibody, p68 antibody, 2600009A06Rik antibody, Hlr1 antibody, wu:fa56a07 antibody, wu:fb11e01 antibody, wu:fb16c10 antibody, wu:fb53b05 antibody, ddx5 antibody, DEAD-box helicase 5 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 antibody, DEAD (Asp-Glu-Ala-Asp) box helicase 5 antibody, DEAD-box helicase 5 L homeolog antibody, DDX5 antibody, Ddx5 antibody, ddx5 antibody, ddx5.L antibody

Background: DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp/Glu/Ala/Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.Synonyms: DDX-5, DEAD box protein 5, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68

Molecular Weight: 69.1 kDa according to NP_004387

Gene ID: 1655

NCBI Accession: NP_004387

UniProt: P17844

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus