LCP2 antibody (N-Term)

Catalog No. ABIN374443

Product Details anti-LCP2 Antibody

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This LCP2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-ALRNVPFRSE V

Specificity: This antibody recognizes LCP2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_005556.

Application Details

Application Notes: Peptide ELISA: 1/32,000. Western Blot: 0.5-2 μg/mL. Detects a band of Approx 75 kDa (Predicted Molecular Weight65 kDa). Positive Control: Jurkat lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for LCP2

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Alternative Name: LCP2 (LCP2 Products)

Synonyms: AI323664 antibody, BB161688 antibody, SLP-76 antibody, SLP76 antibody, twm antibody, Slp76 antibody, lymphocyte cytosolic protein 2 antibody, Lcp2 antibody, LCP2 antibody

Background: LCP2 is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.Synonyms: LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76

Gene ID: 3937

NCBI Accession: NP_005556

UniProt: Q13094

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway