This antibody is expected to recognize isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Porcine, Bovine. Species reactivity (tested):Human, Rat.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen
Peptide with sequence from the C Terminus of the protein sequence according to NP_006229.1. Genename: PPARD
PPARD
Reactivity: Human
WB, FACS
Host: Rabbit
Polyclonal
RB31677
unconjugated
Application Notes
Peptide ELISA: 1/16000. Western blot: 0.3 - 1 μg/mL Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
PPARD is a member of the nuclear hormone receptor subfamily. Upon activation, PPARD forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. PPARD controls genes involved in lipid metabolism, and enhances fatty acid catabolism and energy uncoupling in adipose tissue and skeletal muscle. PPARD has also been implicated in the proliferation and differentiation of preadipocytes, enterocytes, keratinocytes and the placenta. PPARD agonists could be used therapeutically to target multiple components of the metabolic syndrome, including insulin resistance, hyperglycemia, dyslipidemia, and atherosclerosis.Synonyms: NR1C2, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, PPAR-beta, PPARB, PPARD, Peroxisome proliferator-activated receptor delta