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Caspase 4 antibody (Internal Region)

CASP4 Reactivity: Human WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN374466
  • Target See all Caspase 4 (CASP4) Antibodies
    Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))
    Binding Specificity
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 58
    • 33
    • 15
    • 14
    • 1
    Human
    Host
    • 78
    • 10
    • 1
    • 1
    • 1
    Goat
    Clonality
    • 77
    • 13
    Polyclonal
    Conjugate
    • 53
    • 15
    • 10
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This Caspase 4 antibody is un-conjugated
    Application
    • 74
    • 29
    • 27
    • 12
    • 9
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB)
    Sequence
    CKERAEEIYP IKERN
    Specificity
    This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP-150649.1).
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
    Immunogen
    Peptide from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.
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    Discover our top product CASP4 Primary Antibody
  • Application Notes
    Peptide ELISA: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 40 kDa band observed in Human Heart lysates(calculated MW of 43.0 kDa according to NP_001216.1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
  • Target
    Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))
    Alternative Name
    Caspase-4 (CASP4 Products)
    Synonyms
    ICE(rel)II antibody, ICEREL-II antibody, ICH-2 antibody, Mih1/TX antibody, TX antibody, Casp11 antibody, CASP13 antibody, CASP1 antibody, CASP4 antibody, Caspl antibody, ich-3 antibody, Casp4 antibody, caspase 4 antibody, caspase 4, apoptosis-related cysteine peptidase antibody, caspase-4 antibody, CASP4 antibody, Casp4 antibody, LOC100731032 antibody
    Background
    Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67 % homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX
    Gene ID
    837, 9606
    UniProt
    P49662
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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