EHD2 antibody (C-Term)

Catalog No. ABIN374469

Product Details anti-EHD2 Antibody

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This EHD2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to EH domain-containing protein 2 and is expected to recognise EHD1 protein as well.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 0.003 - 0.01 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EHD2

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Alternative Name: EHD2 (EHD2 Products)

Synonyms: PAST2 antibody, BC027084 antibody, C130052H20Rik antibody, fj66d02 antibody, wu:fj66d02 antibody, zgc:77671 antibody, MGEPS antibody, MGC64266 antibody, EHD1 antibody, MGC107790 antibody, EH domain containing 2 antibody, EH-domain containing 2 antibody, EH-domain containing 2b antibody, EH domain containing 2 L homeolog antibody, EHD2 antibody, Ehd2 antibody, ehd2b antibody, ehd2.L antibody, ehd2 antibody

Background: EHD2 is homologous to human EHD1. The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71 % , 86 %, and 76 %, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus. An EHD2 transcript has been detected by northern blot analysis that is highly expressed in heart and moderately expressed in placenta, lung and skeletal muscle, there is another transcript that is expressed at low levels in heart and skeletal muscle.Synonyms: EH domain-containing protein 2, PAST homolog 2, PAST2

Gene ID: 30846

NCBI Accession: NP_055416

UniProt: Q9NZN4

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development