HSD11B1 antibody (C-Term)

Catalog No. ABIN374504

Product Details anti-HSD11B1 Antibody

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Binding Specificity: C-Term

Reactivity: Human, Sheep, Cow, Chimpanzee

Host: Goat

Clonality: Polyclonal

Conjugate: This HSD11B1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CTSYNMDRFI NK

Specificity: This antibody is expected to recognise Human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence from the C-Terminus of Human HSD11B1 (NP_005516.1, NP_861420.1) Genename: HSD11B1

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 0.3 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-HSD11B1 antibody (ABIN374504)

Liu, Guo, Wang, Zhu, Li, Mi, Myatt, Sun: "Inhibition of Lysyl Oxidase by Cortisol Regeneration in Human Amnion: Implications for Rupture of Fetal Membranes." in: Endocrinology, Vol. 157, Issue 10, pp. 4055-4065, (2017) (PubMed).

Target Details for HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Alternative Name: 11-beta HSD1 / HSD11B1 (HSD11B1 Products)

Synonyms: 11-DH antibody, 11-beta-HSD1 antibody, CORTRD2 antibody, HDL antibody, HSD11 antibody, HSD11B antibody, HSD11L antibody, SDR26C1 antibody, hsd11 antibody, hsd11b antibody, LRRGT00065 antibody, hydroxysteroid 11-beta dehydrogenase 1 antibody, hydroxysteroid (11-beta) dehydrogenase 1b antibody, hydroxysteroid (11-beta) dehydrogenase 1 L homeolog antibody, HSD11B1 antibody, HSD11B1b antibody, hsd11b1.L antibody, Hsd11b1 antibody

Background: HSD11B1 is a microsomal enzyme that catalyses the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyse the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.Synonyms: 11-DH, 11-beta-hydroxysteroid dehydrogenase 1, Corticosteroid 11-beta-dehydrogenase isozyme 1, HSD11, HSD11L

Gene ID: 3290

NCBI Accession: NP_001193670

UniProt: P28845

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process