Peptide ELISA: Detection Limit Dilution 1/16000. Western blot: 0.01-0.03 μg/mL. Approx 22 kDa band observed in Human Kidney lysates(calculated MW of 22.2 kDa according to NP_005555.2). Immunohistochemistry on Paraffin Sections: 2-3 μg/mL. In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleatedcells, and of nucleoli in some of the non-dividing cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances.Synonyms: 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, NGAL, Neutrophil Gelatinase-associated Lipocalin, Oncogene 24p3, p25