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Nanog Homeobox (NANOG) (Internal Region) antibody

Details for Product No. ABIN374591, Supplier: Log in to see
Antigen
  • 2410002E02Rik
  • ecat4
  • ENK
Epitope
Internal Region
28
24
22
21
20
17
14
13
13
13
12
9
8
8
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Human
331
89
35
7
3
2
2
2
1
Host
Goat
218
103
41
2
Clonality
Polyclonal
Conjugate
Un-conjugated
16
16
15
15
12
12
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
274
202
110
71
60
53
38
37
22
13
8
4
4
3
2
1
1
1
1
Supplier
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Immunogen Peptide from the internal region of the protein sequence according to NP_079141.2.
Sequence C-QNQRMKSKRW QKNN
Specificity This antibody is specific to NANOG.
Cross-Reactivity (Details) Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Pig.
Purification Ammonium sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name NANOG (NANOG Antibody Abstract)
Background Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG
Gene ID 79923, 9606
UniProt Q9H9S0
Application Notes Peptide ELISA: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 38 kDa band observed in Human Ovary lysates(calculated MW of 34.8 kDa according to NP_079141.1). Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stemcells are stained for Nanog (green). (See also Ref.2)This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Nanog Homeobox (NANOG) (Internal Region) antibody (ABIN374591) anti-Nanog Homeobox (NANOG) (Internal Region) antibody
 image for anti-Nanog Homeobox (NANOG) (Internal Region) antibody (ABIN374591) anti-Nanog Homeobox (NANOG) (Internal Region) antibody (Image 2)
Product cited in: Montserrat, Ramírez-Bajo, Xia, Sancho-Martinez, Moya-Rull, Miquel-Serra, Yang, Nivet, Cortina, González, Izpisua Belmonte, Campistol: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, 2012 (PubMed).

Vassena, Montserrat, Carrasco Canal, Aran, de Oñate, Veiga, Izpisua Belmonte: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, 2012 (PubMed).

Montserrat, Bahima, Batlle, Häfner, Rodrigues, González, Izpisúa Belmonte: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, 2011 (PubMed).

Montserrat, Garreta, González, Gutiérrez, Eguizábal, Ramos, Borrós, Izpisua Belmonte: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, 2011 (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena, González, Barrero, Veiga, Raya, Izpisúa Belmonte: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, 2010 (PubMed).

Raya, Rodríguez-Pizà, Guenechea, Vassena, Navarro, Barrero, Consiglio, Castellà, Río, Sleep, González, Tiscornia, Garreta, Aasen, Veiga, Verma, Surrallés, Bueren, Izpisúa Belmonte: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, 2009 (PubMed).

Aasen, Raya, Barrero, Garreta, Consiglio, Gonzalez, Vassena, Bili?, Pekarik, Tiscornia, Edel, Boué, Izpisúa Belmonte: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes." in: Nature biotechnology, Vol. 26, Issue 11, pp. 1276-84, 2008 (PubMed).