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PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Internal Region)

Details for Product anti-PDPK1 Antibody No. ABIN374625, Supplier: Log in to see
Antigen
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • pdpk1
  • MGC82080
  • zgc:153787
  • CD79B
  • LOC100304995
  • Pdk1
  • PDPK2
  • PRO0461
  • Phosphoinositide-dependent kinase 1
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase-1a
  • CD79b molecule, immunoglobulin-associated beta
  • CD79b
  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1
  • Protein PDK-1
  • Pdk1
  • pdpk1
  • pdpk1a
  • CD79B
  • CD79b
  • LOC100304995
  • Pdpk1
  • PDPK1
  • pdk-1
Alternatives
anti-Human PDPK1 antibody for Western Blotting
Epitope
Internal Region
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34
31
27
23
21
18
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15
15
15
15
8
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6
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3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
435
216
216
18
14
10
10
8
6
3
2
2
2
1
1
1
1
1
1
1
1
Host
Goat
359
68
15
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
17
14
11
11
7
7
7
7
7
7
7
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5
5
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4
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3
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1
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1
Application
Western Blotting (WB)
287
152
93
91
60
59
38
27
20
10
7
5
3
2
1
Options
Supplier
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Immunogen Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence
Specificity This antibody reacts to PDK1.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Canine, Porcine, Bovine.
Species reactivity (tested):Human, Rat.
Purification Affinity chromatography
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Synonyms: Pyruvate dehydrogenase [lipoamide] kinase isozyme 1 mitochondrial, Pyruvate dehydrogenase kinase isoform 1
Gene ID 5163
NCBI Accession NP_002601
UniProt Q15118
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development
Application Notes Peptide ELISA: Detection Limit: 1/1000. Western Blot: 1-3 μg/mL. Approx 65-70 kDa band observed in Rat Heart lysates (calculatedMW of 49.0 kDa according to NP_002601.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0,5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Internal Region) (ABIN374625) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Internal Region) antibody
Background publications Sarbassov, Guertin, Ali, Sabatini: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).