14-3-3 sigma/SFN antibody (Internal Region, N-Term)

Catalog No. ABIN374638

Product Details anti-14-3-3 sigma/SFN Antibody

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Binding Specificity: Internal Region, N-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-DLHTLSEDSY KDST

Specificity: This antibody recognizes 4-3-3 sigma/Stratifin.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Porcine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006133.1.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.1-0.3 μg/mL. Approx.28 kDa band observed in Human Skin lysates (calculated MW of 27.8 kDa accordingto NP_006133.1). Immunohistochemistry on Paraffin Sections: 2-3 μg/mL. In paraffin embedded Human Skin, this antibody shows strong nuclear staining in selectedcells of the basal layer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: 14-3-3 Protein sigma / SFN (SFN Products)

Synonyms: YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody

Background: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions.Synonyms: Epithelial cell marker protein 1, HME1, Stratifin

Gene ID: 2810, 9606

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction