NONO antibody (C-Term)

Catalog No. ABIN374683

Product Details anti-NONO Antibody

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NONO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-NRAAPGAEFA PNK

Specificity: This antibody recognizes NONO / NMT55.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence (NP_031389.3). Genename: NONO

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Alternative Name: NONO / NMT55 (NONO Products)

Synonyms: NMT55 antibody, NRB54 antibody, P54 antibody, P54NRB antibody, nmt55 antibody, nrb54 antibody, p54nrb antibody, xp54nrb antibody, AA407051 antibody, AV149256 antibody, nonA antibody, non-POU domain containing octamer binding antibody, non-POU domain containing, octamer-binding antibody, non-POU domain containing, octamer binding L homeolog antibody, non-POU-domain-containing, octamer binding protein antibody, NONO antibody, Nono antibody, nono.L antibody

Background: Nmt55/p54nrb is a DNA and RNA binding protein that has been implicated in multiple cellular functions such as regulation of cell division as well as cancer biology. Expression of this protein has been found to be decreased in some human breast tumors, and has been studied as a relevant tumor marker. It has been found that decreased nmt55/p54nrb expression in Estrogen Receptor (ER)-negative human breast tumors or the expression of nmt55/p54nrb variants in ER-positive tumors may indicate the loss of normal growth. It is possible that nmt55/p54nrb acts as a pre-mRNA splicing factor affecting the structure and function of hERalpha.Synonyms: NRB54

Gene ID: 4841

NCBI Accession: NP_001138880

UniProt: Q15233