Serotonin Receptor 3A antibody (Internal Region)

Catalog No. ABIN374713

Product Details anti-Serotonin Receptor 3A Antibody

Target: Serotonin Receptor 3A (HTR3A)

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Sequence: C-GPQDFEKSPR DR

Specificity: This antibody is expected to recognize all three reported isoforms (NP_998786.2, NP_000860.2, NP_001155244.1).

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence Genename: HTR3A

Application Details

Application Notes: ELISA: 1/128000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Serotonin Receptor 3A antibody (ABIN374713)

do Amaral, Ueno, Oliveira, Borsonello, Vanzela, Ribeiro, Alves, Barbosa, Carneiro, Boschero: "Reduced expression of SIRT1 is associated with diminished glucose-induced insulin secretion in islets from calorie-restricted rats." in: The Journal of nutritional biochemistry, Vol. 22, Issue 6, pp. 554-9, (2011) (PubMed).

Lu, Hsieh, Dun, Oprea, Wang, Luo, Lin, Chang, Lee: "Estrogen rapidly modulates 5-hydroxytrytophan-induced visceral hypersensitivity via GPR30 in rats." in: Gastroenterology, Vol. 137, Issue 3, pp. 1040-50, (2009) (PubMed).

Target Details for Serotonin Receptor 3A

Target: Serotonin Receptor 3A (HTR3A)

Abstract: HTR3A Products

Synonyms: MGC52789 antibody, HTR3A antibody, 5-HT3 antibody, 5HT3R antibody, HTR3 antibody, 5-HT-3 antibody, 5-HT3A antibody, 5-HT3R antibody, 5HT3 antibody, Htr3 antibody, 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic L homeolog antibody, 5-hydroxytryptamine receptor 3A antibody, 5-hydroxytryptamine (serotonin) receptor 3A antibody, htr3a.L antibody, HTR3A antibody, Htr3a antibody

Background: 5-hydroxytryptamine (serotonin) receptors is a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The 5HT3A receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.Synonyms: 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3A, Serotonin-gated ion channel receptor

Gene ID: 3359

NCBI Accession: NP_000860

UniProt: P46098

Pathways: Synaptic Membrane